In the last decades, Colombian agriculture has been affected by the reduction in productivity in horticultural areas, increase in production costs and the dependence of chemical products usage, causing and irreversible damage to environment and producers and consumers life quality. The aim of this research was to evaluate the effect of plant growth promoting rhizobacteria of the genus Pseudomonas sp. under Lactuca sativa cultivar White Boston as phosphate rock solubilizing bacteria. The study took place in the Research Centre Tibaitata-Corpoica, located in Mosquera (Cundinamarca-Colombia). Results demonstrated that the strains have an intrinsic capacity to solubilize low solubility phosphorus sources as phosphate rock. Inoculants application based on: Pseudomonas fluorescens FR1, Pseudomonas sp., UVLO27 and Pseudomonas sp. LEAV18 strains displayed the best results. The strains Pseudomonas sp. FR2, UVLO27 and K35, are capable of producing indoles and siderophores. The green house experiment showd that strain Pseudomonas fluorescens FR1, Pseudomonas sp. FR2 of phosphate rocks represents a viable economic and ecological alternative in sustainable agriculture systems.