ImpactU Versión 3.11.2 Última actualización: Interfaz de Usuario: 16/10/2025 Base de Datos: 29/08/2025 Hecho en Colombia
ESTUDIO DE LA ACTIVIDAD ENZIMÁTICA DE POLIGALACTURONASA EN LA CORTEZA DE PITAYA AMARILLA (Acanthocereus pitajaya) Polygalacturonase Activity in Yellow Pitaya Peel (Acanthocereus pitajaya)
Polygalacturonase activity was determinated in yellow pitaya fruit (Acanthocereus pitajaya). Protein concentrations and activities of the enzyme in the extracts were determined. Buffer sodium phosphate 20 mM pH 7,0 with NaCl 0,5 M was the most effective extraction system. The enzyme exhibited optimum activity at pH 5,0 in buffer citrate at 40 °C. The KM of the enzyme was 2.9 mg/mL for polygalacturonic acid and VMAX of 0.076 nmol of reducing groups/s. Results show that polygalacturonase is related with softening on yellow pitaya fruit.
