Vitrinite maceral flotation kinetic constants are important in designing flotation columns to be used in separating this maceral group. This work shows the effect of pH, air speed and froth concentration on vitrinite maceral flotation kinetic constants by using two Colombian bituminous coals: El Cerrejon (Guajira) and La Jagua (Cesar). A 5 m high pilot-scale flotation column was used in all runs. The samples were processed using 4-10 pH, 0.7-2.1 cm/s air speed and 2-6 ml/kg of coal froth concentration ranges. Both La Jagua and El Cerrejon coals showed high kinetic constants figures in acid conditions (0.692 min -1 at pH 4 and 0.559 min -1 at pH 5, respectively). The results showed that, generally, the best operating conditions for obtaining high vitrinite maceral kinetic constant values were having an acid medium and low air flow and froth concentration.