Backgraund: S. aureus infections are a public health problem by the profile of antimicrobial multiresistance possessing. Objective: To characterize the resistance phenotype in S. aureus strains isolated from patients treated at Hospital Rosario Pumarejo de Lopez at the city of Valledupar. Materials and methods: 50 strains of S. aureus were analyzed to determine their pattern of susceptibility to different antimicrobials. Results: 12 resistance phenotypes were found and 8 of them with a pattern of multiple resistance to more than three antimicrobials. 50 % of S. aureus were resistant to methicillin and were isolated more frequently from wound samples (30 %) and emergency areas (14 %) and surgery (10 %). Besides oxacillin, antibiotics which had a higher percentage of resistant in vitro were penicillin (98 %), tetracycline (20 % Kirby Bauer and 30 % microdilution broth), clindamycin, and erythromycin (12 % Kirby Bauer and 16 % microdilution broth) and ciprofloxacin (8 % Kirby Bauer and 10 % microdilution broth). Any Staphylococcus introduced resistance phenotype inducible to clindamycin and penicillin. Conclusion: High resistance to oxacillin was found, suggesting the importance of implementing greater epidemiological surveillance to monitor the prevalence of this pathogen in our environment because of the entailed clinical implications.