ImpactU Versión 3.11.2 Última actualización: Interfaz de Usuario: 16/10/2025 Base de Datos: 29/08/2025 Hecho en Colombia
Modificación de un protocolo estándar de extracción de ADN para flebotominos pequeños (Phlebotominae: Lutzomyia) Modification of a standard protocol for DNA extraction from smaller sandflies (Phlebotominae: Lutzomyia)
We evaluated the yield of a potassium acetate protocol with minor modifications for the extraction of genomic DNA. The results showed that using the modified protocol gave a higher DNA yield (0.61, and concentration 37.34 ng/µL) was obtained with respect to the original protocol. The amplification of DNA by PCR for mitochondrial regions demonstrated the high quality of this DNA for molecular population studies, in less time and cost, without the use of highly toxic reagents.
