Background: In Colombia, cervical cancer is the most common type of cancer among women and is the second leading cause death by cancer. In the last five years there have been between 490,000 and 500,000 new cases of female carriers of the Human Papilloma Virus, of which between 300,000 and 220,000 will evolve to become cervical cancer and around 243,000 will end in death. A number of studies have shown the fundamental role of viral genotype in the evolution of cervical cancer. Objective: To genotype the human papillomavirus in a population of sexually active female affiliates of a health promotion entity in Barranquilla between the months of January and December of 2011. Materials and Methods: A cross-sectional study with multiple variables, using as a source of 100 samples of epithelial tissue product of biopsies from patients whose cytology and colposcopy results were positive for high- grade neoplasms. Results: Of the 100 samples where the existence of the virus was confirmed, 45 were of genotype HPV 16, 28 of HPV genotype 18, 10 HPV genotype 33 and it was less frequently found the HPV 6, 11, 26, 31, 35, 39, 42, 45, 54, 58, 59 and 66, which are recorded in the Caribbean Region especially in Barranquilla. Conclusion: The genotypes found with greater frequency in the sample are HPV 16 and 18, while the ones with lower frequency correspond to others that have previously been reported in the region.