The coffee bean is object of researches in our country since this represents a greater percentage of Colombian exports Several studies have focused on field research, soil survey, pest management, spraying, among other. But there are few researches about coffee beans at the microscopic level, In this paper is the analysis of the vibrational structure of comercial coffee beans, by spectrometric techniques Raman microscopy in situ and Fourier Transform Infrared Spectroscopy (FTIR) for determination of its organoleptic.