ImpactU Versión 3.11.2 Última actualización: Interfaz de Usuario: 16/10/2025 Base de Datos: 29/08/2025 Hecho en Colombia
Modificaciones técnicas en el uso de microsatélites y AFLP para el estudio poblacional de diversas especies de peces en el río Sinú, Colombia Modified techniques used for microsatellite and AFLP for the population study of divers species at the Sinu river fish, Colombia
Few genetic conservation studies carried out in Colombia have applied molecular biology techniques. Protocols and modifications arising from the genetic study of four fish species from the Sinu river basin are presented as guidelines for future work in this field (and with other species) as laboratory standardisation processes are complex. The original commercial kit’s DNA extraction protocols were modified as were those for PCR reactions for obtaining micro-satellite markers and AFLPs, as well as allele genotypification. Particular variations were made for each of the species studied.