In order to determine the effect that the vinasse application has on P fractions and their relationship to the accumulation of total biomass in sweet corn, a greenhouse experiment was performed with soils from the municipalities of Florida (Inceptisol Typic Argiudoll with intrusions Entic Dystropept) and Palmira (Mollisol Fluventic Haplustoll) in Valle del Cauca, Colombia. A completely randomized design with four treatments and five replications was used. The treatments were applied trying to supply the potassium requirements of growing corn and separated into two periods: 15 days after sowing and, before crop flowering. The evaluation of phosphorus fractions was performed at the beginning and