The production of cellulases and hemicellulases enzymes represents an important biotechnological potential for various industries of paper, textiles, biofuels, agriculture, among others. These enzymes are useful because they catalyze the processes of mineralization of recalcitrant polymers that are present in the agro-industrial waste. Actinomycetes are the microbial model group in the production of secondary metabolites and their ability to produce cellulases and hemicellulases has been scientifically tested, these enzymes degrade the components of the plant cell wall. Five strains with cellulolytic activity (MCR-33, MCR-24, MCR-9, MCR-4 y MCR-2) were selected, these strains had been previously tested on carboxymethyl cellulose. In this study, the selected strains were identified through their 16SrRNA genes, subsequent PCR were made to identify genes encoding enzymes such as endoglucanases, ?-glucosidases, arabinofuranosidases and xylanases. The identification of these five strains determined all of them belong to the Streptomyces genus, also a gene encoding the ?-1,4-xylanase enzyme was identified in the Streptomyces strains MCR-33 y MCR-24. Furthermore, the presumptive identification of a gene encoding an arabinofuranosidase enzyme which is part of the glycoside hydrolases family was made in the strain MCR-9 and the amplification of the gene encoding the ?- glucosidase enzyme was achieved in the Streptomyces strains MCR-33, MCR-24, MCR-9 y MCR-2. These results reflect the great diversity and potential use of microorganisms present in our country.