The increasing environmental pollution, the presence of chemical residues in foods of animal origin and the development of anthelmintic resistance, caused by improper use of antiparasitic compounds, demand the search for non-chemical alternatives for the control of bovine gastrointestinal nematodes. One of them is the biological control using nematophagous fungi. To overcome the unsustainability of the traditional parasite control methods, the present study was carried out, aiming to: isolate and identify, from samples of soil, fungi that showed ability to trap L3 larvae of gastrointestinal nematodes of cattle; to evaluate in vitro their nematophagous ability; to determine whether or not they were capable of withstanding an artificial digestion process and if they survived the passage through the gastrointestinal tract of cattle. Soil samples obtained from farms in Cundinamarca and Boyaca were processed. Six isolates were identified: four of Arthrobotrys oligospora and two of Arthrobotrys musiformis. Nematophagous capability of the fungi, in vitro, was evaluated by exposing gastrointestinal nematode L3 larvae to concentrations of 1x106 conidia of A oligospora or chlamydospores of A. musiformis, the latter showing the highest percentage (94.56%) of larvae catching. Neither viable conidia nor chlamydospores were observed after artificial digestion; however, A. musiformis chlamydospores were recovered from feces of a calf receiving this species; for this reason, this fungus is postulated as a potential biological agent for the control of gastrointestinal nematodes of cattle in Colombia.