Vascular wilt caused by Fusarium oxysporum is one of the most limiting diseases in the Cape gooseberry crop, with the aim of controlling this disease and to improve crop characteristics of Cape gooseberry a total of twenty isolates: fourteen belonging to the genus Pseudomonas sp. and six belonging to Bacillus sp. were isolated from the rhizosphere of healthy plants of Cape gooseberry in the Combita (Boyaca) and Granada (Cundinamarca) municipalities. These isolates were evaluated for their ability to promote plant growth in Cape gooseberry seeds, phosphate solubilization qualitative and antagonistic activity in vitro. 20 isolates increased the percentage of seed germination of Cape gooseberry, however, only 18 isolates increased root length and stem Cape gooseberry seedlings compared to control, especially the isolates Pf09, Pf11, Pf14, Bs01, Bs02 and Bs03.. The solubilization of phosphate was detected qualitatively in the genus Pseudomonas sp.. Most of the isolates displayed > 30% inhibition of in vitro micelial growth of F. oxysporurm, 63% showed Bs03. Pf02, Pf05, Pf09, Pf11, Pf13, Bs01, Bs02, Bs03, Bs04, Bs05 y Bs06 isolates were selected for improve germination and growth of Cape gooseberry seeds and antagonistic activity against F. oxysporum.