In the synthesis of bacterial cellulose have used various carbon sources, various configurations and strategies of bioreactor cultivation. In this study we compared the production of bacterial cellulose evaluating the effect of different concentrations of fructose in the production of bacterial cellulose (BC) by Gluconacetobacter xylinus IFO static culture. The study was carried out in bioreactors of 750ml cylinder, 200 ml of medium containing initial concentrations of 1, 5, 10, 15 % p/v of fructose at pH 5.6. The inoculum volume was 10% v / v of culture medium volume. Cellulose synthesis was performed at room temperature and at different incubation times ranging from 1.5 to 28 days, which time is determined on the physicochemical and mechanical properties of bacterial cellulose, as well as fructose consumption versus time for different initial concentrations of fructose. The results indicate that 10% w/v fructose highest value obtained for the synthesis of BC in grams per liter of moist cellulose culture medium and the maximum thickness was obtained with a volume decrease of remnant; furthermore, fructose consumption observed until 90% of the initial concentration. The compressibility was less than a greater thickness of the cellulose film. The medium pH was kept in a region for optimal synthesis of BC.