Studies on a protease as an efficient, environmental friendly and relatively economical remover of residual proteins for historical valuable documents were performed and was selected for this work, from the filamentous fungi collection of the Bogota Archive. 32 morphotypes of 74 evaluated show hydrolytic activities over differential solid media. From them, 8 morphotypes obtained directly from documental samples and representative of the genera Penicillium and Stachybotrys were selected and their isoenzyme profile were tested. Also 2 previous morphotypes with promisorius proteolytic activities and representative of the genera Chaetomium and Eladia were analysed. Extracelullar proteases production was induced in differential liquid media on three fermentation steps and analitycal isoelectrofocusing were performed over pH 3.0-10.0 ranges. Only the morphotypes 8D (Chaetomium sp.), and 21D (Eladia sp.), showed an alkaline isoform with pIs 8.5 and 8.8,