Colombian Squirrels cytogenetics showed a great variability which has renewed the interest in evolutionary aspects within the group. Many chromosome banding tools must be analyzed carefully in addition to the classical G-banding G technique. These techniques include other differential bands like Q and R banding and nondifferential banding (C and NOR). In this article the use of each of these supplements in the cytogenetic analysis of species and cytotypes observations for the Columbian squirrels is explained.