Beauveria bassiana strain 9205 (Bb9205) was transformed with protease genes (pr1A and pr1J ) and one sterase gene (ste 1) isolated from fletartiizium anisopliae that are involved in insect pathogenicity. The goal of this research was lo increase the pathogenicity of the strain against the coffee berry borer. !iypothenemus hampei. To do this, genes were cloned into the plasmid pBarOF’El that confers resistance to the herbicide ammonium-gluphosinate. A monosporic une of strain Bb9205 was obtained with pathogenicity of’ 78% mortality against 11. hampei, using 1 x 106 spore/ml. Protoplasts were produced from this monosporic line. The methodology for the protoplast production was optimized obtaining 3 x 10 protoplasts/ml after enzymatic pretreatment for 4 hours under slow agitation at 37°C. ‘flEansformation was carried out with the plasmids pBarGPEl- pr1A, pBarOPEI-priJ and pBarGPF.1-ste 1 by PEO and electroporation. Two strains transformed with the pr1A gene and one strain transformed with the .ste 1 gen were obtained, that showed resistance to 25 itg/ml of ammonium glufosinate. These strains showed increased proteolytic and sterolytic activity, respectively. Under laboratory conditions, pathogenicity tests of 13. bassiana transformants was compared to untransformed monosporic 13b9205. Constitutive over-expression of protease in the transgenic strain Bb9205-pr 1A improved its insecticidal activity. The transformed strain showed a 21,7% increase in the insect mortality rate with 14,3% reduction in the time of mortality. The Bb9205-ste 1 transformant strain showed a 9,5% reduction of the time of mortality compared with the untransforrned strain. The results indicate that breeding of 13eauveria strains by genetic transformation can be achieved for purposes of better biological control.