The present objective this study was to identify genera of nematodes associated with colosuana grass (Bothriochloa pertusa (L) A. Camus) in municipality of Sampues of Sucre department. The isolation of nematodes from roots and soils were performed by the Baermann funnel technique and their identification was made by using existing taxonomic keys to nematodes. We identified 10 genera of nematodes found in greater proportion Cephalobus gender (bacterivore) with respect to Pratylenchus, Criconemella (phytoparasitic) Dorylaiminae (omnivore), Prionchulus and Tripyla (Predators), Aphelenchoides (micofago) Tylocephalus, Acrobeles and Rhabditis (Bacterivoros). This is the first report that we have in the colombian Caribbean diversity of nematodes associated with soil and grass roots colosuana.