Sugarcane harvesting residues are considered as a raw material for fuel ethanol production due its high content of cellulose, around 40% DS. The use of cellulose depends of the application of physicochemical or biochemical treatments that allow the release of glucose and its subsequent uses in fermentation processes. The enzymatic hydrolysis of these residues requires a cellulolytic complex produced by microorganisms, including three enzymatic activities: Endoglucanases, β-Glucosidases and Exoglucanases. In the present study, cellulolytic enzymes produced by two native fungi Aspergillus spp., CH 2016 and CH 2001 was assessment, through of solid-state fermentation processes using as raw substrate RAC and pre-treated with organosolvents (delignified). Strain CH 2016 had the highest endoglucanase activity 11.0773 U/mL in the raw substrate on day seven of fermentation, the same strain, in the delignified substrate showed the highest activity