We describe a new species of the Anolis aequatorialis group from the central Andes of Colombia. The new species, Anolis anoriensis, is similar to A. eulaemus Boulenger, which occurs in both the western and central Andes, and was positioned in the eulaemus subgroup of the aequatorialis group. Anolis anoriensis differs from A. eulaemus in having smaller interparietal scales and a green body coloration with a darker anterior part of the dewlap. We also for the first time describe the coloration of Anolis eulaemus, which is almost exclusively brown with a diffused light brown dewlap.