The sea cucumber Stichopus sp. is a species that inhabits the Colombian Caribbean, and little is known about their reproductive behaviour under controlled laboratory conditions. During 2012 and 2013, from July to October, spontaneous spawning and spermiation events took place in the Aquaculture Laboratory of the Universidad del Magdalena (Santa Marta, Colombia). The mean number of fertilised eggs was 48.4 x 10 6 , which developed up to the late auricularia stage. In this study, handling conditions, water quality and limitations associated with reproduction and larval culture are described.