Summary Dictyochromenol an unusual compound, rarely found in plants, and two known compounds obtained from petroleum ether extract of the whole plant Piper tricuspe showed antimalarial and antioxidant activity as well as cytotoxicity. The results show that the compounds are active against several Plasmodium falciparum strains with IC50´s ranging from 1.37 to 29.78 µM and cytotoxic effects with IC50´s ranging from 1.07 to 40.95 µM; the selectivity index based on IC50´s suggests a high toxicity of the compounds. In addition, the compounds showed high antioxidant activity with IC50´s ranging from 10.8 to 165.6 µM in the DPPH assay. Compounds were characterized by MS, 1 H and 13 C NMR and identified as Dictyochromenol, 3-Farnesyl-p-Hydroxy benzoic acid and 2´E,6´E 2-Farnesyl Hydroquinone. The ethnomedical claim of Piper tricuspe in the Colombian Pacific zone may be justified by the observed antiplasmodial activity of the extracts.