This article is part of a research on prejudice, stereotyping and discrimination among college students. This study was aimed to understand students' perceptions of the University of Narino toward their Afro-Colombian and Indigenous peers. The study was developed from the qualitative paradigm through focus groups with students from Pasto and Ipiales headquarters. Four categories were taken into account: personality, interpersonal relationships, academic performance and cultural tradition. The results show that there are positive and negative stereotypes towards the analyzed communities, presenting higher negative stereotypes towards Afro-Colombian community. It was founded that the poor academic performance and the outgoing personality trait are relevant factors among Afro-Colombian students, and in regard to the indigenous students, it stands out their appreciation for their cultural values and their introversion. These stereotypes affect the coexistence between students from the mentioned communities as well as the ones who don't belong to them.