The process of multiplication of arbuscular mycorrhizae fungi (AMF) from indigenous banana agro- environments from Uraba (Antioquia - Colombia) was evaluated, using solid substrate, with different 'host' plants and substrates, and the effectiveness of the samples obtained from banana plants (Musa AAA cv. 'Gran Enano'). The average association of the HMA to the plants was 37,76 ± 21,86%, regarding this percentage, the plants 'B' (Brachiaria decumbens) and 'S' (Sorgum vulgare) favored gratly the association. Considering the substrate, the 'S2' (sand 50-soil 50) and the 'S6' (Vermiculite 50-soil 50) had associations significantly superior to treatments. In plants 'S' and 'K' (Pueraria phaseoloides) and in substrate S1 (sand 30-soil 70), was found a higher number of spores. The combination plant-substrate that most favored the association was the plant B (used as a trap culture) using substrates 'S2' and 'S4' ('cascarilla de arroz' 50-soil 50) and the production of spores were plants 'K' and 'S' in substrate 'S1'. The general HMA association in banana plants coming from tissue crop was 48,74 ± 30,44; there were not found significant differences between plants with zero days and plants with 30 days already acclimated. The samples with inoculum diseases spread upon them which favored the association, were the ones coming from the banana agro-environment compared to the commercial samples and those from natural environments in Uraba. The greatest foliar dry and root weight was found on banana samplings applied with 'I5' (sample from agro-environment). For the growth variables there was not found any difference.