The present study analyzes the cannabinoids content in samples of Cannabis sativa L. cultivated illicitly in Colombia. The physicochemical conditions are optimized for the extraction and quantification of Cannabidiol ( CBD ), 9-Tetrahidrocannabinol ( THC ) and Cannabinol ( CBN ) starting from a vegetable sample using gas chromatography with flame ionization detector (GC-FID), validating the respective analytic method. Samples coming from four diferent Colombian regions are analyzed (Llanos Orientales, LL; Cauca, CA; Sierra Nevada de Santa Marta, SN; Eje Cafetero, EC). The study let determination of CBD , THC and CBN samples quantitatively. The average content of THC in the samples of the region LL is of 15.74% ± 2.92, in the region CA is of 10.98% ± 6.70, in the region SN is of 2.81% ± 1.72 and in the region EC of 1.87 % ± 1.25. The higher content of THC in vegetable samples of Cannabis from the Llanos Orientales (LL) and Cauca (CA) could be indicative of the employment of improved varieties. This fact generates a great concern about the potential effects produced by the drug in consumers.