Background: Calendula officinalis has multiple therapeutic properties such as improving the process of cicatrization. Therefore, it is interesting its possible value and use to treat oral pathologies. Objective: To observe the effect of three presentations of C. officinalis in different concentrations on the proliferation of the human gingival fibroblasts. Methods: Human Gingival Fibroblasts (FGH) in fifth pass were obtained from healthy donor explants and exposed to stimuli with ethanolic extract of C. officinalis (EEC), tincture of C. officinalis (TC) and K-Trix® rinsing in concentrations of 750, 500, 150, 100 μg/ml. Proliferation of the FGH was observed at 12, 24 and 48 hours in relation with a group of cells without getting any stimulation, using the MTA test with Cell Titer 96®. Results: The major proliferative effect was achieved by the EEC in concentrations of 750 and 500 μg/ml at 12 hours. The tincture of 100 and 150 μg/ml C. officinalis at 48 hours generated inhibition in cell growth whereas the rinsing K-Trix® did not have effect on the proliferation of the FGH in any concentration at any time. Conclusion: The proliferation of the FGH depends on the presentation of the C. officinalis , time, and concentration.