Solid substrate fermentation has wide industrial application; nowadays enzymes are mainly used in obtaining dairy products, sweeteners, drugs, food, liquor, detergent, etc. Lignin is enzymatically degraded by Basidiomycetes fungi through the non-specific oxidation of three different types of enzymes: laccase, manganese-peroxidase and lignin-peroxidase. Their non-specificity is most important in degrading different recalcitrant substances showing structural similarity with lignin, such as PAHs, PCBs, DDT, azoic colorants, etc. The research was done using balloon flasks as reactors and seven different agro-industrial waste from the Santander region as substrate (their lignolytic enzyme production was unknown): corn husks, sugar cane pulp, pineapple leaves, cacao and coffee shells, rice husks, wheat bran and wheat straw. The general objective was to evaluate the ability of the Basidiomycetes (Trametes versicolor, Pleurotus floridae and Phanerochaete chrysosporium) to grow on agro-industrial waste and produce lacease, manganese- peroxidase and lignin-peroxidase lignolytic enzymes by solid substrate fermentation. It was found in this work that lignolytic enzyme production in the aforementioned substrates was sensitive to reducing sugar concentration, pH condition, humidity content and depended on the type of inductor present in the culture medium. The substrate presenting the best laccase and manganese-peroxidase production was wheat straw. Lignin-peroxidase production was observed in corn husk and wheat bran cultures.