In the present study, seven new species of Riodinidae from Colombia are described based on material deposited in the authors’ collections and by comparative analysis of literature and revision of other collections in Colombia: Colaciticus seitzi n. sp., Lucillella aphrodita n. sp., Euselasia tenebrosa n. sp., Machaya aenigmatica n. sp., Esthemopsis chocoana n. sp., Adelotypa andresi n. sp., and Symmachia titiana occidentalis n. ssp. New records of thirteen species belonging to the genera Esthemopsis, Themone, Napaea, Cyrenia, Menander, Euselasia, Symmachia, Ithomeis, Nahida, Pachytone and Panaropsis, are also presented and comments on the taxonomic status of each species are provided.