Approximately 60% of the land in Colombia that is planted with oil palm is acidic with a high aluminum saturation of over 50% of the cation exchange capacity. To understand the physiological and biochemical response of oil palm rootlets to aluminum toxicity and to identify possible tolerance mechanisms and the materials best adapted to this stress, six oil palm interspecific OxG hybrids (U1273, U1737, U1757, U1859, U1914, and U1990) were planted in a hydroponic system with three levels of aluminum concentrations (0, 100 and 200 M) in a randomized complete split-plot design with five replications. The root variables of growth, accumulation of aluminum, nutrient content, production of spermidine, and exudation of organic acids were recorded. High levels of aluminum inhibited root growth, altered the absorption of water and nutrients, especially nitrogen, calcium, iron, and manganese, and activated tolerance mechanisms, such as organic acid release, homeostasis of certain essential ions, and spermidine production. These responses were differential between the different hybrids, identifying the U1990 hybrid as the most tolerant to aluminum. Despite having the greatest accumulation of aluminum, the U1990 hybrid produced the longest and most abundant roots in response to the increased production of spermidine and oxalic, malic, and acetic acids and was better able to regulate nitrogen and manganese.