Polyhydroxyalkanoates (PHAs) are biodegradable biopolymers of bacterial origin with properties similar to conventional plastics. In this work, bacteria were isolated from soils contaminated with fique (Furcraea bedinghausii) wastes in the municipality of Guarne (Antioquia) and their ability to produce PHAs was evaluated. Bacteria were stained with Nile blue and Nile red and the PhaC gene detected by PCR. Positive bacteria were identified by phylogenetic analysis of 16S rDNA and biochemical tests. The chemical nature of the biopolimers was determined by gas chromatography GC-MS/SIM using biomass produced in a submerged fermentation in a minimal media supplemented with glucose as sole carbon source. Four bacterial morphotypes identified as Bacillus megaterium (2), B . mycoides (1) and Gordonia sp. (1) showed potential