qPCR detection and quantification of Colletotrichum lindemuthianum in bean tissue and seeds from Antioquia, Colombia Bean anthracnose, a disease caused by Colletotrichum lindemuthianum, is one of the most limiting factors for the bean crop. This pathogen is mainly transmitted from infected seeds making the use of certified seeds vital for its control. Detection of C. lindemuthianum is based on the use of culture media and, more recently, PCR techniques such as qPCR. The latter is one of the methods of choice due to its speed and high levels of specificity and sensibility. In this work, the SYBR Green qPCR technique was tested using primers ClF432/ClR533. Specificity was tested with DNA extracted from mycelia and infected plant tissues (leaves and pods) using a serially diluted DNA control sample as standard curve. The presence of C. lindemuthianum was also verified in commercial and non-commercial seed samples. qPCR detected C. lindemuthianum in eight mycelia samples tested [Threshold Cycle (Ct): 4.3-9.85] and 17 out 18 bean tissue samples (Ct: 16.71-29.38) regardless of the presence of symptoms. The pathogen was detected in 84 % of the whole set of tested seeds, and in 85 % of the commercial samples; however, the amount of DNA detected in the latter was lower than that for non-commercial seeds (1-32.2 pg·µL -1 vs. 1-279.3 pg·µL -1 ). These results