Introduction. The selection assisted by molecular markers allows the modification of the genesfre - quency within a population, favoring the variables responsible for zoo technical interest characters. The gene of the growth hormone is important for the characteristics related to the animals development and can be associated to the linear characteristics of the bovine cattle. Objective. To determine the effect of a polymorphism of the bovines growth hormones gene on conformation characteristics in Holstein cows from the Antioquia province. Materials and methods. The research work was performed in eight dairy herds from Antioquia, with 359 records, with 313 Holstein bovines. The DNA extraction was performed with the salting out method and the ge- notyping was made by means of PCR-RFLP. To evaluate the association between the polymorphism and the linear characteristics, a general linear model was adjusted and the Kruskal Wallis test was used. Finally, a linear regression was performed in order to evaluate the allelic substitution effect by the use of the SAS V.9.0 statistical software. Results. The polymorphism of the growth hormones gene had a significant effect (P 0.05) for the other characteristics analyzed. Conclusion. The