This paper reports the results of a study on genetic and environmental variability between 43 lines of Chonto tomato, Lycopersicon esculentum Mill, without using progeny offspring test. The experimental material were planted under field conditions using three randomized blocks. Data on individual plants were recorded for yield/plant, number of fruit/plant, average fruit weight, number of inflorescences/plant, number of fruits/inflorescence, number of locules/fruit, average locule weight, fruit shape index, pericarp thickness, days to flowering, harvest lenght, plant heigh, first inflorescence heigh, internode lenght, reaction to the presence of Scrobipalpula absoluta and Xanthomonas vesicatoria. The quantitative characters were subjeted to variance analysis in orden to evaluate the genetic and environmental variation. Heritability in the wide sense, genetic variability coeficient and the expected progress by selection were estimated. Fenotipic, environmental and genetic correlations among some evaluated characters were also carried out. Important genotipic differences, at 1% of probability, for the evaluated characters were found. Generally, in the most of the evaluated characters the largest contribution of the total variation correspond to the genetic variation. The contribution of the environmental variation was very low.