RESUMEN La papa ( Solanum sp. ) es el cuarto producto alimenticio mas importante en el mundo. En Colombia anualmente se producen alrededor de 2,8 millones de toneladas, sirviendo como sustento economico a 90.000 familias. En el pais, Tecia solanivora genera el mayor impacto economico en el cultivo con perdidas de hasta el 100% en la produccion de tuberculos. El fitomejoramiento via introduccion de genes Cry , que codifican para cristales proteicos insecticidas, constituye una alternativa para reducir el ataque de insectos en cultivos de interes comercial. En este trabajo se caracterizo la insercion, transcripcion y expresion del gen Cry1Ac en diferentes tejidos y en tres etapas del desarrollo para dos lineas transgenicas de Solanum tuberosum spp. andigena variedad Diacol Capiro. La caracterizacion se realizo a traves de tecnicas de PCR, RT-PCR y ELISA. Se corroboro la insercion y transcripcion del gen utilizando primers que amplificaron una banda especifica de 766pb para Cry1Ac . Los niveles de expresion de la proteina llegaron a ser mayores a 45 μg/g y no mostraron diferencias significativas entre las lineas analizadas, ni entre las tres etapas del desarrollo. No se evidenciaron diferencias significativas entre las lineas transgenicas con respecto al control al hacer un analisis de algunas caracteristicas fenotipicas relevantes. Los resultados encontrados sugieren la realizacion de seguimientos y ensayos de bioseguridad sobre este material, ya que los altos niveles de expresion en todos los tejidos analizados, pueden afectar a organismos no blanco. ABSTRACT Potato is the fourth of the most important crop and foodstuff in the world, in Colombia around 2.8 millions of tons are produced annually supporting economically 90000 families. In the country, the major economic impact in the crop is caused by Tecia solanivora that originates loses up to 100% in the tuber production. The genetic plant breeding related to the introduction of Cry genes which codify insecticidal crystal proteins is an alternative for reducing the insect attack in commercial crops. In this work, the insertion, transcription and expression of Cry1Ac gen was characterized in different tissues and three development stages of two transgenic lines of Solanum tuberosum varieties Diacol Capiro and Parda Pastusa that were transformed with that gen. The characterization was realized by PCR, Rt-PCR and ELISA techniques. The gen insertion and transcription was confirmed using primers for Cry1Ac gen that amplified a specific band of 766 bp. The protein expression levels were higher than 45 μ g/g and were not significantly different between the analyzed lines neither the three development stages. Furthermore, taking in account some relevant phenotypic features, no significant differences were found between transgenic lines and controls. The results suggests monitoring and biosecurity assays with this vegetal material because their high level expression and inside all of tissues analyzes that could affect non-targeted insects. Palabras clave: S. tuberosum , Diacol Capiro, Cry1Ac , caracterizacion molecular, cultivos transgenicos.