The health of building occupants may be affected by bioaerosols, particularly aerosolized bacteria and fungi. We determined airborne bacteria and fungi in 31 settings from a public general hospital. Air samples were taken by the impaction method on solid surface. Microbial identification was run by standard microbiological techniques. Results were interpreted by the criteria from The Spanish Association of Hospital Engineering and Canadian Health and Wealth Department. Results showed microbial density values ranging from 1 UFC/m 3 to 222 UFC/m 3 . In general, bacteria in surgical and non-surgical settings were within the ¨clean¨ range. However, the Oto-rhino M2 surgery room, D surgery room and the Nephrology surgery room exceeded the non-contaminated range. The Sterilization room and the Neonatal intensive care unit also depicted bacteria and fungi contamination, respectively. Coagulase-positive Staphylococcus was found in the Traumatology J surgery room and in the Urology S1 surgery room, while Serratia marcescens was isolated in the Nephrology surgery room. Therefore, J and S1 surgery rooms were also considered contaminated regardless of their low bacterial count. Bacterial identification revealed 14 genera and 8 species, being coagulase-negative Staphylococcus the most frequent bacterial isolate. The majority of the locations showed fungal densities values within the ¨very clean¨ and ¨clean¨ ranges, showing the isolation of 12 genera and 5 species. Aspegillus and Penicillum spp. were the most frequent fungal isolates. The indoor air microbiological quality in white theaters was determined by a rapid cultured-based method and a combination of indoor air microbiological quality criteria.