Hepatozoon spp. es el protozoo intracelular con mayor prevalencia en las serpientes, con presentandose en algunas ocasiones sin cambios metabolicos, sin embargo, en presencia de stress u otras patologias puede contribuir a la mortalidad. En el Centro de Atencion y valoracion de Fauna silvestre del Area Metropoitana del Valle de Aburra, se muestrearon un total de 55 boas (Boa constrictor) que ingresaron al centro en el periodo comprendido entre noviembre de 2006 y junio de 2007. Para el diagnostico de Hepatozoon sp, se tomo una muestra de sangre a nivel intracardiaco, se realizo un extendido coloreado con tincion de Wright y se observo al microscopio. Del total de los animales evaluados se encontraron 17 individuos positivos a la infeccion, representando este valor, una prevalencia de 30,91% boas infectadas con este protozoo. Abstract Hepatozoon spp. Is the intracellular protozoan most prevalent in snakes, appearing on certain occasions without metabolic changes, nonetheless, in the presence of stress or other pathologies it may contribute to their mortality. In the Center for Attention and Appraisal of Wild Animals of the Metropolitan area of the Aburra Valley, a total of 55 boas (Boa constrictor) were sampled, entering the center between November 2006 and June 2007. For diagnosing Hepatozoon sp. a blood sample was taken from inside the heart, a colored extension was carried out using Wright’s tincture and it was observed under the microscope. Of all the animals evaluated, 17 individuals were positive for infection, with this number representing a prevalence of 30,91% of boas infected with this protozoan.