Objective. To determine susceptibility to Metronidazole when isolates Helicobacter pylori obtained from Colombian patients during the period April 2006 to March 2007. Materials and methods. The susceptibility test to Metronidazole was realized using the E Test Method to 53 isolates of H. pylori obtained through cultures of gastric biopsies, coming from adult patients to whom endoscopies of upper gastrointestinal tracts were practiced. Results. Fifty-three isolates of H. pylori were obtained from 26 men and 27 women. The antimicrobial susceptibility E Test method showed that 72% of the isolates of H. pylori were resistant to Metronidazole. Conclusion. Resistance of H. pylori to MTZ currently continues being high and is comparable to the one published 10 years ago (73% and 84% respectively). For being greater than 40%, it is recommended not to use this antimicrobial agent in the Colombian eradication programs.