Used cooking oil for the production of biotechnology products in an alternative for giving added value to this residue andreduce sewage pollution. The Pseudomonas genus is capable of using this oil as a source of carbon and for its metabolismsynthesizes some biosurfactants (biodegradable surfactants). Were evaluated 3 initial concentrations of oil: 10 g/L, 15 g/Land 20 g/L with a carbon ratio of 11,6:1. A production method was established at laboratory level with erlenmeyer andaeration during 72 h and a temperature of 32°C and pH 7,00 in supplemented culture medium with 15 g/L of used cookingoil. The recovery of the biosurfactant (extracellular product) was made by centrifugation, acidification with hydrochloricacid (HCl) and extraction with chloroform: methanol (2:1). Chloroform was recovered using a rotavapor at 40°, obtainingpure biosurfactant. Biosurfactant was characterized as regards to surface tension and contact angle. The minimum surfacetension reached by the biosurfactant was 32 mN/m, the determined CMC by conductivity was 56,72 mg/L and emulsionindex 52,59%. Results achieved indicate that it is technologically possible to obtain biosurfactants from used cooking oil.