In this work, a systematic study on theeffect of 1-butanol, 1,2-butanediol and1,2,3,4-butanetetrol (erythritol) on thesurface tension of β-lactoglobulin inaqueous solution at pH 6.5 and 298.15K is presented.The experimental data were used tocalculate the surface pressure and wereadjusted to different protein adsorptionmodels at the liquid-air interface to explainthe behavior of β-lactoglobulin inaqueous solution. The results show thatthe alcohols produce a significant effecton the adsorption behavior of the proteinat the interface that is related to the numberof hydroxyl groups.