This study describes the validation of multiresidue analytical methodology for the simultaneous determination of 19 organochlorine, organophosphorus and organonitrogen pesticides in strawberry. Pesticides residues were extracted from strawberry samples with ethyl acetate, the extracts were cleaned-up by GPC, the quantitative analysis was carried out by high resolution gas chromatography (GC) with a pulsed splitless injection mode and simultaneous detection by μ -ECD and NPD coupled in parallel.