The main purpose of this study was the standardization of several isozymic systems using clones of different ploidy belonging to the Colombian Collection of Musa maintained under in vitro conditions. A total of 23 systems were assayed. Six isozymes presented new electrophoretic activity with regard to previous reports: Diaphorase (DIA E.C.1.6A.3.), Malic enzyme(ME E.C.1.1.1AO) Phosphoglucoisomerase (PGI E.C.5.3.1.9.), Phosphogluconate dehydrogenase (PGDH E.C.1.1.1.44.), Phosphoglucomutase (PGM E.C.2.7.5.1.) and Rubisco (RUB E.CA.11.1.39.). The enzymes DIA and RUB are reported the first time for the genus Musa. ME, GDH, PGDH and PGI are described the first time in acrylamide support. DIA presented high level of polymorphism in the Colombian clones tested. RUB showed only one zone of activity.