Bone tissue is in a continuous process of synthesis and resorption, functions that are performed by three main cell types: osteblasts, osteocytes and osteoclasts. Osteoclasts (OC) are big, multinucleate cells, producing carbonic anhydrase and tartrate-resistant acid phosphatase (TRAP). In continuation with previous works where CAp was synthesised by precipitation of aqueous solution and by solid state reaction, in this article the results of a culture of osteoclasts and its biological action over low-density polyethylene impregnated with carbonated apatite slides (LDPE-CAp) are shown. Osteoclast induction was achieved with the addition of specific inductors to cultures of porcine bone marrow progenitors, to which TRAP activity, detection of transcripts of rank, c-src and cat-K genes were determined. Cultures on LDPE-CAp slides analyzed with optical and electron microscopy demonstrated osteoclast activity * Correspondencia: Jose Mikan jose.mikan@unimilitar.edu.co. Direccion Correspondencia: Laboratorio de investigaciones, Facultad de Medicina, Universidad Militar Nueva Granada, Tr. 3a #49-00, Bogota, Colombia. Recibido: Mayo 10 de 2010 Aceptado: Junio 14 de 2010. Revista 18 (1): 23-31, 2010