Titulo en ingles: Screening for a fungal protease with potential in the biorestoration of historical valuable documents in Bogota Archive Resumen: Se ha buscado y seleccionado sistematicamente una proteasa que pudiese ser usada en la eliminacion “limpia” de encolantes sobre soportes documentales con valor de patrimonio historico de forma eficiente y economica, a partir de la coleccion de hongos filamentosos del Archivo de Bogota. De 74 morfotipos viables evaluados sobre placas selectivas, 32 morfotipos presentaron formacion de halos de hidrolisis evidentes sobre placas diferenciales. De ellos, se evaluo el perfil isoenzimatico de 8 morfotipos provenientes de muestreos documentales directos y de 2 morfotipos proteoliticos promisorios provenientes de un trabajo previo. Los 10 morfotipos seleccionados fueron representativos de los generos Penicillium , Stachybotrys , Chaetomium , y Eladia . Luego de inducir la produccion de proteasas extracelulares en medios liquidos diferenciales bajo tres fases de fermentacion, se realizaron isoelectroenfoques analiticos tendientes a la observacion de isoformas en el gradiente de pH establecido (3.0-10.0). Solo los morfotipos 8D ( Chaetomium sp.) y 21D ( Eladia saccula ) presentaron una isoforma alcalina extrema, de puntos isoelectricos 8.5 y 8.8, respectivamente, susceptible de seleccion con miras a su purificacion y caracterizacion parcial de forma economica y eficiente. Los demas morfotipos, representativos de los generos Penicillium sp., y Stachybotrys sp., presentaron unicamente isoformas proteoliticas en el rango acido de pH con puntos isoelectricos que oscilan entre 4.0 y 5.0. Palabras clave: biodeterioro; hongo filamentoso; proteasa; halo de hidrolisis; punto isoelectrico. Abstract: Studies on a protease as an efficient, environmental friendly and relatively economical remover of residual proteins for historical valuable documents were performed and was selected for this work , from the filamentous fungi collection of the Bogota Archive. 32 morphotypes of 74 evaluated show hydrolytic activities over differential solid media. From them, 8 morphotypes obtained directly from documental samples and representative of the genera Penicillium and Stachybotrys were selected and their isoenzyme profile were tested. Also 2 previous morphotypes with promisorius proteolytic activities and representative of the genera Chaetomium and Eladia were analysed. Extracelullar proteases production was induced in differential liquid media on three fermentation steps and analitycal isoelectrofocusing were performed over pH 3.0-10.0 ranges. Only the morphotypes 8D ( Chaetomium sp.), and 21D ( Eladia sp.), showed an alkaline isoform with pIs 8.5 and 8.8, respectly, suceptible of selection for its purification and characterization through efficient and economical way. The others morphotypes only showed acid isoforms with pIs in the range of 4.0 and 5.0. Key words: biodeterioration; filamentous fungi; protease; hydrolytic halo; isoelectric point