The sugar cane POJ 2878 is susceptible both to the Fiji disease as the vector (P. saccharicida Kirkaldy) and ocuppies high percentage of the total cane area cultivated in Colombia. It means a real need to develop methods of control to regulate its populations. This study has shown that Metarhizium anisopliae (Metch.) Sorokin C7 isolate was the most pathogenic culture on P. saccharicida at concentrations of 1 x 108 spores/ml. Death rate produced by mycosis were taken beginning the second day for 20 consecutive days. The mortality produced by M. anisopliae C7 on different stages of P. saccharicida were: 10% for first instar, 23.3% for the second, 66.6% for the third 58.6% for the fourth, 66.6% for the fifth and 86.7% for the sixth. Furthermore, several microorganisms associated with P. saccharicida were evaluated in their pathogenicity on the most susceptible stage to M. anisopliae C7. A. Fusarium spp. isolate showed similar pathogenic results.