One hundred and eight euglossine bee species are registered for Colombia, distributed in five genera: Aglae (1), Eufriesea (30), Euglossa (60), Eulaema (13) and Exaerete (4). New records for Colombia include: Eufriesea auripes, Ef. dressleri, Ef. fragrocara, Ef. xantha, Euglossa viridis, Eg. azureoviridis, Eg. cognata, Eg. singularis, EI. boliviensis, EI. seabrai and EI mocsaryi. Keys are given for males and females of Aglae, Exaerete, Eulaema and Eufriesea and for males of Euglossa of the 108 Colombian euglossines. A key for the subgenera of Euglossa is presented by the first time.