The present investigation evaluated the impact of Bioaugmentation and Biostimulation of contaminated sediment with hydrocarbons from the Fuel Service Station INTEGRA Dosquebradas – Risaralda - Colombia, these sediments are the result of the maintaining in treatment units of industrial wastewater, as: the grease trap, perimeter channels of distribution area and/or fuel sale machines, the perimeter channels of storage tanks and vehicle washing, sand trap at the service station. For the development of this research, in the bioaugmentation technique the sediments were added with microorganisms adapted to hydrocarbon of the Service Station, these were incorporated with soil that was contaminated earlier by an oil spill; for biostimulation technique the contaminated sediments were added with Urea fertilizer (CON2H4) and water by a manual disposition, benefiting the development and growth of degraders. This tests used eight (8) mesocosmos, compounds high density polyethylene baskets of 57x37x15 cm, the experimental mesocosmo were formed, first, contaminated sediments from the service station only, second mesocosmo, contains contaminated sediments and were added urea a nutrient, third mesocosmo contains 40% of adapted soil microorganisms, and 60% of contaminated sediments; and the fourth mesocosmo was taken with 40% of adapted soil microorganisms and 60% of contaminated sediments also added urea a nutrient, for all these units were create a replica, resulting in eight (8) mesocosmos in total. For monitoring and control were measured throughout the experiments once a week temperature, pH, and moisture percentage, twice a month total petroleum hydrocarbons, and once a month nitrogen percentage, organic matter percentage, phosphorus, potassium and hydrocarbon degraders. The entire duration of the tests were twenty (23) weeks. The mesocosmos showed degradation rates between 87.32 mg TPH (Total hydrocarbon oil)/kg of dry soil and 105.41 mg TPH/kg of dry soil, with the reduction in content hydrocarbon percentage between 79.7% and 95.1%. The two strategies for bioremediation, biostimulation and bioaumentacion, no statistically significant differences (p> 0.05) (Tables 17 -19).
Tópico:
Microbial bioremediation and biosurfactants
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