This study was conducted in the coffee growing zone of Valle del Cauca (Colombia) to evaluate the effect of planting coffee under different cropping systems: organic, conventional and organic-mineral, on soil phosphorus (P) fractions. Adapted sequential fractionation methodology was done by the International Center of Tropical Agriculture. The statistical analysis consisted of a Complete Randomized Block Design under a split plot arrangement with three treatments and three replications. The results showed that contents of the organic fraction of available P and the moderately available P had significant differences (P > 0.5) among the conventional and organic systems, in which the conventional system had the lowest values. The organic-mineral system showed the highest contents of organic and inorganic P and the conventional system showed the lowest for these fractions of P. For the non-available P fraction, it was found that organic systems had higher P content than the conventional system and, the organic-mineral system showed significant differences with respect to the rest. Total P showed a similar pattern than the fractions described above. The low content in the conventional system can be explained by specific aspects of management such as the use of synthetic chemical fertilizers, planting in monoculture without shade and lack of soil coverage between plants.