An experiment was conducted with 252 laying hens (42 to 62 weeks age) to evaluate the use of three levels (0,25 Y 50 %) of cassava flour (CF) in combination with soy bean meal (SBM) and whole cooked soy bean (WCSB) in the diet. There were no differences (P < 0.05) due to the CF levels; however, production o lo and feed consumption increased, egg weight and yoIk color decreased when increased the levels of CF; the diets with 0% and 50 % CF had respectly the best conversion/dozen egg and conversion/kg egg. There were differences (P < 0.05) with a best response in feed consumption, egg production, egg weight, feed consumption/kg egg production, egg weight, feed consumption/kg egg produced and yolk color due to the use of WCSB replacing SBM in diets containing milo and C F for laying hens. The interaction level of cassava x protein source was not significant (P < 0.05). Economically the best net benefic was obtained with the diet containing 0 % of CF (100% milo) + WCSB.