The preliminary checklist of Chiribiquete flora has 549 species of vascular plants, which belong to 315 genera and 107 families. The families with the highest species number were: Rubiaceae (32), Melastomataceae (31), Orchidaceae (25) and Bromeliaceae (24); there were 30% of the families represented by one species. The majar tloristic affinities of the Chiribiquete flora are with the Amazonregion and the Guayana central province. Eleven species (including ten new) are endemic to Chiribiquete and 167 are endemic to the Guayana region.