Although the capacity of forests and some silvopastoral systems to store carbon is well known, there is little information about their potential to sequester carbon in soil and tree biomass in the main land uses in landscapes dominated by cattle. We estimated the storage of soil organic carbon (SOC) and tree biomass carbon in different land uses in Colombia, Costa Rica and Nicaragua. The land uses evaluated were degraded pasture, native pasture and improved pasture with and without trees, fodder bank, forest plantation, riparian forest and secondary forest. Soil samples were taken to determine SOC and the bulk density was measured. The amount of C stored in soil was adjusted by soil mass. Carbon in tree biomass was estimated using temporal plots where diameter at breast height was measured, and the carbon stored was calculated using allometric equations. The secondary forest was the land use with more carbon stored (both in soil and trees) in Costa Rica and Nicaragua; in Colombia, it was the riparian forest. Degraded pasture was the land use with less carbon in all three countries. Our results show that in the three agricultural landscapes studied, degraded pastures had significantly lower C stocks compared to improved pastures with trees and forest systems, and that there are good opportunities for C sequestration in agricultural landscapes dominated by cattle. 1 Grupo Ganaderia y Manejo del Medio Ambiente, CATIE. Correo electronico: fcasasol@catie.ac.cr 2 Fundacion CIPAV, Colombia. Correos electronicos: cesar@cipav.org.co, jnaranjo@cipav.org.co 3 Instituto Nitlapan, Universidad Centroamericana, Managua, Nicaragua.