Objective: To know the reproducibility of results of the WHO-5 WBI among nine-grade adolescent students in Cartagena (Colombia). Method: A total of 157 students participated in the research with mean age of 15.1 (SD = 1.1), 56.1 % were boys. WHO-5 WBI consists of five items that quantify depressed mood, decreased interest in activities and energy lost during the last two weeks. It was calculated Cronbach alpha and omega McDonald coefficients as indicators of internal consistency; and Pearson correlation coefficient (r) and intraclass correlation coefficient (ICC) as estimators of the stability. Results: WHO-5 WBI showed acceptable internal consistency in the first and second application, Cronbach's alpha of 0.668 and 0.697, respectively, and omega of McDonald and 0.686 0.671, respectively. Similarly, WHO-5 WBI showed high stability over four weeks with Pearson coefficient (r = 0.612, P <0.001) and ICC 0.613 (95% CI 0.505-0.702). Conclusions: The WHO-5 WBI presents acceptable reproducibility over four-weeks among adolescent students. It is needed to know the psychometric performance of the WHO-5 WBI using the best gold standard.